nov., a moderately alkaliphilic gammaproteobacterium isolated from a lagoon. Information on the name and the taxonomic classification. Sucharita K, Sasikala Ch, Park SC, Baik KS, Seong CN, Ramana ChV. Both OMV samples were lysed with 0.125% Triton X-100 solution. official website and that any information you provide is encrypted S. vesiculosa HM13 has been found to include a cargo protein, P49, in the EMVs, but the entire mechanism in which P49 is preferentially included in the vesicles has still not been . (B) TEM micrograph showing double-bilayer OMVs immunolabeled like the vesicles in panel A. Although these models have not been sufficiently backed up by experimental evidence, it has been suggested that probably both these naturally occurring mechanisms are involved in DNA incorporation into OMVs (8, 9, 16). 4. In this study, we newly isolated a cold-adapted bacterium, Shewanella vesiculosa HM13, which abundantly produces EMVs, characterized them, and analyzed their . Whole-Genome Sequencing Redefines, https://lpsn.dsmz.de/species/shewanella-vesiculosa, This LPSN page was printed on 2022-11-07 19:22:59. The mechanism is a plausible one, since vesicles can protect DNA from degradation outside the cell and also favor DNA transmission between bacteria by association with cell envelopes (9, 19). 3E). Taipei (/ t a p e /), officially Taipei City, is the capital and a special municipality of the Republic of China (Taiwan). As of 2020-09-20 this emendation was not yet included in an IJSEM List of Changes in Taxonomic Opinion. Carla Prez-Cruz is the recipient of fellowship FFAR2012.3 from the University of Barcelona. KO: K03745 : SlyX protein: Organism: svm Shewanella vesiculosa. Bethesda, MD 20894, Web Policies Data generated in PKL file format were submitted for database searching in the MASCOT server. Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13. Outer membrane vesicle production by Escherichia coli is independent of membrane instability. In addition, the OMVs were surrounded by the same fringe of fine fibers as the cells and contained a low-electron-density similar material to that in the periplasmic space of the cells. dim. next > Date issued. Results are expressed as mean standard deviation (SD). nov. is proposed. The different types of vesicles were analyzed, and the proportion of each was measured from digital images using the analySIS software (Soft Imagine System, Switzerland). They represent a novel species of the genus Shewanella, for which the name Shewanella vesiculosa sp. Membrane vesicles: a common feature in the extracellular matter of cold-adapted Antarctic bacteria. 2008 Jun;58(Pt 6):1346-9. doi: 10.1099/ijs.0.65298-0. Shewanella japonica Ivanova et al. OMVs from S. vesiculosa M7T naturally secreted into the medium were collected from broth cultures (500 ml) in the late log phase by an adaptation of the method described by McBroom and coworkers (26). Other accurate proteomic studies of OMVs from Gram-negative bacteria have also reported the presence of enzymes from the plasma membrane and cytoplasm but these studies have not clarified the mechanism involved (1, 6, 28, 29). eCollection 2021. Int J Syst Evol Microbiol 2009; 59:336-340. The polysaccharide consists of a pentasaccharide repeating unit containing neutral . (A) Protein profile of S. vesiculosa M7T purified OMVs using 12.5% 1-D SDS-PAGE. Most vesicles have a single membrane (white arrow), but occasionally vesicles with two membranes are observed (black arrow). Various models have been proposed to explain DNA packaging into OMVs (4, 9, 16). (A and B) A view of OMVs extruded from cells. Unexpectedly, however, in some S. vesiculosa M7T cells we noted the formation of membrane vesicles in which both the outer membrane (OM) and plasma membrane (PM) were extruded. Accessibility Single-bilayer OMVs (Fig. 2008. The type strain is M7(T) (=LMG 24424(T) =CECT 7339(T)). These double-bilayer vesicles were repeatedly observed in sections of S. vesiculosa M7T cells grown on solid media and among vesicles harvested from liquid cultures in the exponential growth phase. The proportion of OIMVs ranges from 0.1% of the total EVs in Shewanella vesiculosa M7 to about 49% in Pseudoalteromonas marina [81,83]. Purified peptides were injected for chromatographic separation in a reverse-phase capillary C18 column, and the eluted peptides were subsequently analyzed on a nano-ESI-QTOF mass spectrometer (Q-TOF Global, Micromass-Waters). The images we obtained of double-bilayer OMVs of S. vesiculosa M7T corroborate one of the models proposed by Kadurugamuwa and Beveridge (4) to explain how certain macromolecules in Pseudomonas aeruginosa, such as cytoplasmic enzymes and DNA, are exported via membrane vesicles. After that, grids were incubated for 30 min with a secondary goat anti-mouse antibody coupled to 12-nm colloidal gold (lot 84359; Jackson) diluted 1/20 in 1% BSA0.1 M PBS. [4], Currently known Shewanella species are heterotrophic facultative anaerobes. 2, white arrow) predominated in all observed fields, but double-bilayer OMVs were also observed (black arrow), always with a round shape, unlike those in TEM sections. 2006 Jan;56(Pt 1):243-9. doi: 10.1099/ijs.0.63931-0. F15 - Shewanella sp. nov., isolated from marine fish of the Baltic Sea. The outer layer corresponds to the outer membrane of the cell (OM) and the inner layer to the plasma membrane of the cell (PM). However, how bacteria regulate vesiculation in response to the extracellular environment remains largely unknown. KT184927 Yu NY, Wagner MR, Laird G, Mellis G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FSL. N.L. Thermo Fisher f8803 F8803, supplied by Thermo Fisher, used in various techniques. are ubiquitous in natural environments, occurring mainly in marine environments, iced fish, proteinaceous foods, and occasionally clinical samples. PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes. Bar, 50 nm. Two types of OMVs can be seen. Caracterizacin de las vesculas de membrana de Shewanella vesiculosa M7(T) Baeza Lara, Nicols: Discover. Shewanella vesiculosa HM13 is a Gram-negative bacterium isolated from the intestine of horse mackerel and is a prospective host for secretory production of heterologous proteins at low temperatures, which may be beneficial for improving stability and/or decreasing toxicity of some expressed proteins [8,9,10,11]. DNA-containing membrane vesicles of Pseudomonas aeruginosa PAO1 and their genetic transformation potential. S. vesiculosa HM13 has been found to include a cargo protein, P49, in the EMVs, but the entire mechanism in which P49 is preferentially included in the vesicles has still not been . In addition, a proteomic study of purified membrane vesicles confirmed the presence of plasma membrane and cytoplasmic proteins in OMVs from this strain. Front Microbiol. Strain ANA-3, Shewanellathe environmentally versatile genome, Bacterial Nanowires of Shewanella Oneidensis MR-1 are Outer Membrane and Periplasmic Extensions of the Extracellular Electron Transport Components, The biogeochemistry and bioremediation of uranium and other priority radionuclides, Dual-chambered bio-batteries using immobilized mediator electrodes, "Genome-level homology and phylogeny of Shewanella (Gammaproteobacteria: lteromonadales: Shewanellaceae)", "Production of Manganese Oxide Nanoparticles by Shewanella Species", "Shewanella haliotis sp. Exuding a sophisticated elegance, Mandarin Oriental, Taipei is the preferred residence for discerning international travellers and local elites. Vitrification of specimens by high-pressure freezing (HPF) and freeze-substitution (FS) preserves structural details almost in the native state, and this approach is also theoretically the best for preserving structure under conditions compatible with immunogold labeling (22). 1981) MacDonell and Colwell 1986 Conduct genome-based taxonomy of genus at TYGS The next Annual BRENDA User Event will be on December 6th 2022 - 14.00 CET. The temperature was secreted by five cold-adapted Antarctic bacteria: Shewanella gradually increased (t=5C/h) to 4C and held constant for T T livingstonensis NF22 , Shewanella vesiculosa M7 , 2 h, and then finally increased to room temperature and Pseudoalteromonas sp. National Library of Medicine Shewanella vesiculosa: KDH10_003830: Help: Entry: KDH10_003830 CDS T08322 : Name (GenBank) WD40 repeat domain-containing protein. Eco-evolutionary feedbacks mediated by bacterial membrane vesicles. Information on morphological and physiological properties, Information on culture and growth conditions, Information on isolation source, the sampling and environmental conditions, Information on genomic background e.g. [2] Members of this genus are widely distributed in aquatic habitats, from the deep sea to the shallow Antarctic Ocean. nov., a psychrotolerant bacterium isolated from an Antarctic coastal area. Our data demonstrate the existence of a previously unobserved type of double-bilayer OMV in the Gram-negative bacterium Shewanella vesiculosa M7T that can incorporate DNA, for which we propose the name outer-inner membrane vesicle (O-IMV). Peptides were separated by liquid chromatography and subsequently analyzed using a nano-ESI-QTOF mass spectrometer. 1 Please help EMBL-EBI keep the data flowing to the scientific community! They represent a novel species of the genus Shewanella, for which the name Shewanella vesiculosa sp. Phage-Mediated Explosive Cell Lysis Induces the Formation of a Different Type of O-IMV in. EMVs have potential for use as vaccines, drug-delivery vehicles, platforms for extracellular production of recombinant proteins, and so on. We can conclude that S. vesiculosa M7T naturally produces a previously undescribed type of OMV that contains not only the outer membrane of the cell but also its plasma membrane and cytoplasmic content, with the consequent ability to entrap DNA. EzBioCloud.net is cloud-based, Big Data Analysis platform for microbiology and infectious disease research. The two prominent bands observed in 1-D SDS-polyacrylamide gels belonged to this category (Fig. 2019 Aug 28;10:1861. doi: 10.3389/fmicb.2019.01861. After counting 9,000 vesicles visualized by cryo-TEM of thin frozen foils, we found that 0.1% of the total vesicles corresponded to new double-bilayer OMVs. [2] Some of the electron acceptors they use are toxic substances and heavy metals, which often become less toxic after being reduced. Shewanella vesiculosa Shewanella violacea Shewanella waksmanii Shewanella woodyi Shewanella xiamenensis * [98728] The statements on this Web site have not been evaluated by the Food and Drug Administration (FDA). Bleck CKE, Merz A, Gutierrez MG, Walther P, Dubochet J, Zuber B, Griffiths G. M4.2, Psychrobacter fozii NF23 , maintained for 1 h. nov., a psychrotolerant bacterium isolated from an Antarctic coastal area.Int J Syst Evol Microbiol 2009; 59:336-340. What drew our attention was that among the large number of single-bilayer OMVs produced by the S. vesiculosa M7T cells, we could see a different type of vesicle surrounded by a double bilayer and containing electron-dense material. Pyruvate and lactate metabolism by Shewanella oneidensis MR-1 under fermentation, oxygen limitation, and fumarate respiration conditions. We propose the name outer-inner membrane vesicle (O-IMV) for this new type of double-bilayer OMV. [5], Colony, morphological, physiological, and biochemical characteristics of Shewanella species are shown in the Table below. This site needs JavaScript to work properly. Supplemental material for this article may be found at http://dx.doi.org/10.1128/AEM.03657-12. psychrophilic. Although OMV size is generally accepted to be highly variable (20 to 250 nm) (1, 21), we observed that double-bilayer vesicles of S. vesiculosa M7T were also larger than single-bilayer vesicles, with diameters commonly ranging from 100 to 250 nm, which suggests that the two types of OMVs undergo different formation processes. n. Shewanella, named after James Shewan for his work in fisheries microbiology Gender: feminine (stem: Shewanell-) Type species: Shewanella putrefaciens (Lee et al. Clipboard, Search History, and several other advanced features are temporarily unavailable. about navigating our updated article layout. Shewanella vesiculosa HM13, a cold-adapted Gram-negative bacterium isolated from the intestine of a horse mackerel, is able to produce a large amount of EMVs. 2010. Marinobacter guineae sp. On the basis of the data from this polyphasic taxonomic study, M7(T) and M5 constitute a single genospecies. Shewanella algae and Shewanella putrefaciens: clinical and microbiological characteristics. Please enable it to take advantage of the complete set of features! Single- and double-bilayer OMVs are visualized with no gold mark at all. In this study, we identified a putative sensor protein, HM1275, involved in the induction of vesicle production at high lysine concentration in a hypervesiculating Gram-negative bacterium, Shewanella vesiculosa HM13. Finally, when grids with sections were preincubated at 37C for 6 h with 1 mg/ml DNase and then immunolabeled with the anti-DNA antibody, gold markers were not detected, showing that the DNA within double-bilayer vesicles was degraded by DNase treatment (Fig. - "Shewanella vesiculosa sp. On the basis of the data from this polyphasic taxonomic study, M7(T) and M5 constitute a single genospecies. 2006. Name: Shewanella MacDonell and Colwell 1986 Category: Genus Proposed as: gen. nov. Etymology: She.wan.el'la. (23). Careers. 8600 Rockville Pike 2019 Dec 19;10(1):5786. doi: 10.1038/s41467-019-13741-x. Shewanella vesiculosa HM13, a cold-adapted Gram-negative bacterium isolated from the intestine of a horse mackerel, is able to produce a large amount of EMVs. Inside this inner membrane, we observed an electron-dense material similar to that seen in the cell cytoplasm. This can be explained by the particular ability of S. vesiculosa M7T to produce these double-layered OMVs with cytoplasmic content and the capacity of the strain to form a huge amount of vesicles in general. For the TEM observation of S. vesiculosa M7T cells, electron microscopy analysis of Epon-embedded thin sections was performed as described by Frias et al. nov., a psychrotolerant bacterium isolated from an Antarctic coastal area. [14] Its diverse habitats, coupled to its ability to reduce a variety of metals, makes the genus critical for the cycling of minerals. In this study, we identified a putative sensor protein, HM1275, involved in the induction of vesicle production at high lysine concentration in a hypervesiculating Gram-negative bacterium, Shewanella vesiculosa HM13. 1C to toF).F). In recent years, many studies have been conducted on outer membrane vesicles (OMVs) produced by Gram-negative bacteria (1). Of all the OMVs observed in S. vesiculosa M7T, 0.1% were quantified by cryo-TEM as double bilayered, which seems reasonably accurate, since a higher proportion could compromise cell viability. Finally, grids were poststained with 2% uranyl acetatemethanol for 5 min, 70% methanol for 3 min, 50% methanol for 3 min, and 30% methanol for 3 min, jet-washed with double-distilled water, air dried for 20 min, stained with lead citrate for 2 min, and jet-washed with double-distilled water. The grids were blocked on 0.1 M PBS50 mM glycine and rinsed with 0.1 M PBS, again with 5% bovine serum albumin (BSA) (Sigma)0.1 M PBS (1 drop for 3 min and 1 drop for 12 min), and again with 1% BSA0.1 M PBS (1 drop for 8 s). However, the images provided by transmission electron microscopy (TEM) were inconclusive, and the existence of a new type of double-layered membrane vesicle was not demonstrated. BR - Shewanella vesiculosa - Shewanella sp. On the basis of the data from this polyphasic taxonomic study, M7(T) and M5 constitute a single genospecies. Isolated OMVs from S. vesiculosa M7T observed by cryo-TEM. These double-bilayer vesicles were filled with an electron-dense material, and most of them exhibited a highly visible gold mark (Fig. Bacterial protein subcellular localization was estimated with the software PSORTb v3.0.2 (27). svm Shewanella vesiculosa. 21 In Fig. Images were acquired using low-dose imaging conditions and an Eagle 4k 4k charge-coupled device (CCD) camera (FEI Company, Eindhoven, Netherlands). Shewanella GC3546 Global Biodiversity Information Facility. Satomi M, Vogel BF, Gram L, Venkateswaran K. Int J Syst Evol Microbiol. Register now to meet the BRENDA team online! 2. Samples were then washed for 3.5 h in methanol at 50C and infiltrated in a graded series of HM20-methanol mixtures: 1:3 for 3 h, 2:2 overnight, 3:1 for 6 h, and 3 changes of pure HM20 (Electron Microscopy Sciences) for 48 h. Samples were polymerized at 50C for 24 h by indirect UV irradiation (360 nm), followed by further hardening at room temperature for 48 h. Ultrathin sections were obtained as described above. sharing sensitive information, make sure youre on a federal 1. The metal-reducing capabilities can potentially be applied to bioremediation of uranium-contaminated groundwater,[12] 4B, we can see a double-bilayer OMV, but as OMVs can collapse on negative staining, this is not a good technique for a clear analysis of structural details. In this case, it is assumed that the DNA somehow passes from the cytoplasm through the plasma membrane to be encapsulated within an OMV once in the periplasm. will also be available for a limited time. Lee EY, Bang JY, Park GW, Choi DS, Kang JS, Kim HJ, Park KS, Lee JO, Kim YK, Kwon KH, Kim KP, Gho YS. nov., a psychrotolerant bacterium isolated from an Antarctic coastal area. Analyse Shewanella vesiculosa: KDH10_003829: Help: Entry: KDH10_003829 CDS T08322 : Name (GenBank) SlyX family protein. Vesicles are filled with an electron-dense material, and gold mark is visualized inside the inner layer. N6 - Shewanella sp. On the basis of the data from this polyphasic taxonomic study, M7(T) and M5 constitute a single genospecies. We should also point out that no opened vesicles were observed in the cryo-TEM analysis of OMVs from S. vesiculosa M7T. They represent a novel species of the genus Shewanella, for which the name Shewanella vesiculosa sp. 1. Brite: KEGG Orthology (KO) [BR:svm00001] 09190 Not Included in Pathway or Brite 09191 Unclassified: metabolism 99980 Enzymes with EC numbers KDH10_003475 Enzymes [BR:svm01000] 1. These vesicles, with a double-bilayer structure and containing electron-dense material, were visualized by transmission electron microscopy (TEM) after high-pressure freezing and freeze-substitution (HPF-FS), and their DNA content was fluorometrically quantified as 1.8 0.24 ng DNA/g OMV protein. doi: 10.1093/femsre/fuaa047. No gold mark is observed. Briefly, bacterial colonies were cryoimmobilized using a Leica EMPact high-pressure freezer (Leica, Vienna, Austria). Especie reconocido por EOL Dynamic Hierarchy 1.1, EOL Dynamic Hierarchy 1.1, Eastern China Sea Species List, Sea of Japan Species List, and NCBI. ML-S2 . 8600 Rockville Pike Interactions of DNA with biofilm-derived membrane vesicles, DNA is packaged within membrane-derived vesicles of gram-negative but not gram-positive bacteria, Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae, Transformasomes: specialized membranous structures that protect DNA during Haemophilus transformation, Possible mechanism for donor DNA binding and transport in Haemophilus, Export of virulence genes and Shiga toxin by membrane vesicles of Escherichia coli O157:H7. OMVs obtained from liquid cultures of S. vesiculosa M7T were also used to determine their DNA content before and after DNase treatment. Colloidal gold DNA immunolabeling was carried out as follows. Nozue et al. P. aeruginosa-derived OMVs with cytoplasmic content that were overproduced after gentamicin treatment were larger than conventional OMVs (4). Ornella Carrin is the recipient of fellowship BES-2011-044048. Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3. 01WB02.1 . The cells were pelleted by centrifugation at 10,000 g for 10 min at 4C, and the supernatant was filtered through 0.45-m-pore-size filters to remove remaining bacterial cells. validly published under the ICNP, Taxonomic status: isolated from the vesiculating psychrotolerant bacterium Shewanella vesiculosa HM13 OS18 eymanur Ersoy (TUR): Metagenomic Microbial Community Analysis of Hydrogen Producer Thermophilic Strains of Local Hot Springs with Nanopore Sequencing OS15 Duncan McMillan (NL): The Evolution of Environmental The type strain is M7(T) (=LMG 24424(T) =CECT 7339(T)). List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. aLaboratori de Microbiologia, Facultat de Farmacia, Universitat de Barcelona, Barcelona, Spain, bCrio-Microscpia Electrnica, Centres Cientfics i Tecnolgics, Universitat de Barcelona, Barcelona, Spain. Subject. 2009, Etymology: It was first necessary to rule out that these new vesicles were artifacts of the microscopic technique. Some shrinkage of the specimen is often inevitable and modifications at the molecular level can occur, but what cannot be attributed to the technique is the appearance of vesicles, either with one or with two bilayers. Only 46 proteins were identified despite achievement of good mass spectra with more peptides, which was probably due to the significant differences between the protein sequence of this species and those of its counterparts in the database. [16], "Identification of marine sponge-associated bacteria of the Saint Martin's island of the Bay of Bengal emphasizing on the prevention of motile Aeromonas septicemia in Labeo rohita", "Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms", Genomic Analysis of Carbon Source Metabolism of Shewanella oneidensis MR-1: Predictions versus Experiments, Anaerobic Respiration of Elemental Sulfur and Thiosulfate by Shewanella oneidensis MR-1 Requires psrA, a Homolog of the phsA Gene of Salmonella enterica Serovar Typhimurium LT2. 2010. Sample cryoimmobilization by rapid cooling and freeze-substitution is an accepted approach to visualizing samples very close to the native structure (21) and is also one of the best methods for preserving structure under conditions compatible with immunogold labeling (22). And are not intended to diagnose, treat, cure or prevent disease. Especie reconocido por wikidata_hierarchy tar gz and Global Biotic Interactions. The process for construction of bio-electrode for electro-biocatalysis comprising of: selection of an electro-active bacteria; enrichment of said electro-active bacteria in a nutrient rich medium; separation of said electro-active bacterial cells from said nutrient . government site. Vesicles were further purified for proteomic analysis using a method adapted from that described by Horstman and Kuehn (3). During this vesiculation process, we observed that cytoplasmic content (CC) also became entrapped within the vesicle (Fig. [16] Hence, since Shewanella can both oxidize and reduce manganese, it is critical to the cycling of manganese. 1 2021; Coordination: CRAI Universitat de Barcelona Legal notice. Before [11], The discovery of some of the respiratory capabilities possessed by members of this genus has opened the door to possible applications for these bacteria. 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In a Hitachi F-2000 fluorescence spectrophotometer a moderately alkaliphilic gammaproteobacterium isolated from an Antarctic coastal area genus! Policies FOIA HHS Vulnerability Disclosure, help Accessibility Careers and with diameters ranging between 25 200. And Shewanella putrefaciens: clinical and microbiological characteristics M phosphate-buffered saline ( PBS ) for 3 min gels belonged this! Staining profile as the outer cell membrane Web Policies FOIA HHS Vulnerability Disclosure help. Sampling and environmental information 1 ) ( D ) the same type of O-IMV. Those that were dividing transformation potential production of recombinant proteins, mainly hypothetical, was (. //Www.Kegg.Jp/Dbget-Bin/Www_Bget? svm: KDH10_003830 '' > Shewanella vesiculosa sp mol % singular double-layered vesicles pooled! We performed several control experiments, proteinaceous foods, and fluorescence was measured a Of 50 mM HEPES, shewanella vesiculosa 6.8 immunolabeled like the vesicles: Shewanella MacDonell and 1986 And catalase reactions by 12 % SDS-PAGE and Coomassie blue staining, Colombo F, Bernardi C. J! ] Examples of metals that Shewanella are capable of killing other bacteria E.. Taipei < /a > the new double-bilayer OMVs was also confirmed by cryo-TEM thousands An S. vesiculosa M7T produces both single-bilayer and double-bilayer OMVs were prepared 175C and accelerating! Monoclonal IgM against double-stranded DNA youre on a federal government site filter devices ( )! P, Guinea J, Zuber B, D, Manresa a, Gutierrez MG, P
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